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Micromate thread4/24/2023 (1) how quickly the image becomes blurred as it goes out of focus, and In general, DOF is determined by two very different factors: I've been studying DOF for 50 years, and writing about it for more than 10, and I have never figured out how to explain it thoroughly yet concisely. You have my sympathy about the difficulty of good references. Thanks in advance for your guidance and expertise! What aperture should the tube lens be set at, does it matter? Can I shift magnification on the tube lens by rotating the focus ring from one extreme to another? Will moving the focus ring impact the quality of the images being captured? How does having a microscope objective combined with the 200mm tube lens play in the formulas that calculate DoF?īeyond Depth of Field, I'm also wondering about a few other things. Nikon Nikkor Micro 200mm f/4 ED-IF, used as tube lens Over time I'll more than likely add similar objectives with 2x, 5x and 20x magnifications. Soon I'll be acquiring a Mitutoyo M Plan APO 10x objective. I'm hopeful that someone can help me figure out how much overlap I'll need between images to increase stacking success. While I wait for the adapter ring to arrive I've been trying to find a resource that would help me understand and calculate DoF for various combinations I'm envisioning. Recently I purchased an adapter ring to mount Mitutoyo objectives to the front. I have a Nikon Nikkor Micro 200mm f/4 ED-IF and will be using it as my tube lens. I have a few questions and concerns about Depth of Field (DoF) calculations for my soon to be real setup.
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